RNA-Triggered Protein Cleavage and Applications by the CRISPR Cas7-11-Csx29 Complex
The type III-E CRISPR–Cas7-11 effector binds a CRISPR RNA (crRNA) and the putative protease Csx29 and catalyzes crRNA-guided RNA cleavage. We report cryo–electron microscopy structures of the Cas7-11–crRNA–Csx29 complex with and without target RNA (tgRNA), and demonstrate that tgRNA binding induces conformational changes in Csx29. Biochemical experiments revealed tgRNA-dependent cleavage of the accessory protein Csx30 by Csx29. Reconstitution of the system in bacteria showed that Csx30 cleavage yields toxic protein fragments that cause growth arrest, which is regulated by Csx31. Csx30 binds Csx31 and the associated sigma factor RpoE (RNA polymerase, extracytoplasmic E), suggesting that Csx30-mediated RpoE inhibition modulates the cellular response to infection. We engineered the Cas7-11–Csx29–Csx30 system for programmable RNA sensing in mammalian cells. Overall, the Cas7-11–Csx29 effector is an RNA-dependent nuclease-protease.
Researchers
-
rna-triggered protein cleavage and applications by the crispr cas7-11-csx29 complex
United States of America | Pending -
rna-triggered protein cleavage and applications by the crispr cas7-11-csx29 complex
Patent Cooperation Treaty | Published application
License this technology
Interested in this technology? Connect with our experienced licensing team to initiate the process.
Sign up for technology updates
Sign up now to receive the latest updates on cutting-edge technologies and innovations.